Imaging Center Instrumentation
• Zeiss LSM 510 META laser scanning confocal microscope
• Coherent Mira 900 femtosecond laser system
• Warner Instruments dual channel heat controller
• PowerMac G5 workstation
– Deconvolution package: Volocity
– 3D visualization and analysis packages: Volocity, Igor Pro
• HP Pavilion Linux/Windows workstation
– Deconvolution packages: Huygens Professional, Volocity
– 3D visualization and analysis packages: Huygens Professional, Volocity, Igor Pro
Imaging Center Rates
• Zeiss LSM 510 META Confocal Microscope: $72/hr (6/1/06 pending final approval)
• PowerMac G5 Workstation: N/A
• AMD AthlonX2/Linux Workstation: N/A
Imaging Capabilities
• Confocal microscopy on fixed tissues and cells
– tissue sections < 80 μm recommended
• Confocal microscopy on live tissues and cells
– environmental controls available for solution temperature and flow
– tissue sections < 80 μm recommended
• Multiphoton imaging of fixed tissues and cells
– tissue sections up to 400 μm possible
• Multiphoton imaging of live tissues and cells
– environmental controls available for solution temperature and flow
– tissue sections up to 400 μm possible
• Multiphoton uncaging (spatially resolved release of caged compounds)
• Fluorescence recovery after photobleaching (FRAP)
• Spectral imaging in confocal or multiphoton modes
• Spectral unmixing
– simultaneous imaging of multiple fluorophores with overlapping
emission spectra
– online unmixing or post-acquisition modes available
• Ratiometric imaging
– online acquisition or post-acquisition processing available
• Transmitted and reflected light imaging modes
• Non-descanned detection for imaging experiments requiring high-sensitivity
Time-series imaging available for the imaging modes described above